... an understanding of additionandsubtraction concepts and strategies; • providing opportunities to connect subtraction to addition through problem solving; Learning About AdditionandSubtraction ... involving the addition, subtraction, multiplication, and division of singleand multidigit whole numbers, and involving the additionandsubtraction of decimal numbers to tenths and money amounts, ... models and instructional strategies that support student understanding of additionand subtraction; • sample learning activities dealing with additionandsubtraction for Grades 4, 5, and A glossary...
... Examine the MAC table again a Look at the MAC address table again using the show mac-address -table command at the privileged EXEC mode prompt as follows: ALSwitch#show mac-address -table b How many ... show mac-address -table command? c Show only the mac-address-tables that were learned dynamically d How many are there? _ Step Clear the MAC address table To remove ... addresses use the clear mac-address -table command from the privileged EXEC mode prompt as follows: ALSwitch#clear mac-address -table dynamic 2-7 CCNA 3: Switching Basics and Intermediate Routing v 3.0...
... Examine the MAC table again a Look at the MAC address table again using the show mac-address -table command at the privileged exec mode prompt as follows: ALSwitch#show mac-address -table b How many ... mac-address -table command? c Show only the mac-address-tables that were learned dynamically d How many are there? _ 2-7 CCNA 3: Switching Basics and Intermediate ... the MAC address table To remove the existing MAC addresses use the clear mac-address -table command from the privileged exec mode prompt as follows: ALSwitch#clear mac-address -table dynamic Step...
... L M and van den Bergh, J C J M., 2009b Climate change and hailstorm damage: empirical evidence and implications for agriculture and insurance, forthcoming Botzen, W J W., Aerts, J C J H and van ... transnational and compounded crises such as terrorism (Norris et al., 2003; Papacharissi and de Fatima Oliveira, 2008); pandemics (Buus and Olsson, 2006; Shih et al., 2008; Ungar, 2008) and climate ... W., van den Bergh, J C J M and Bouwer, L M., 2009a Climate change and increased risk for the insurance sector: a global perspective and an assessment for the Netherlands Natural Hazards, forthcoming...
... difficulties configuring interfaces or the routing protocol, refer to the Configuring Host Tables lab Step Login to Router and verify the connection to Router a Login to the Gadsden router b Verify the connection ... the ping is not successful, return to Step and troubleshoot the configuration Step Telnet to a remote router a Enter telnet router-name if IP host tables were configured Otherwise, enter the ... both the serial and the FastEthernet interfaces up? _ Step Suspend the current Telnet session a Enter Ctrl+Shift+6 followed by the x key This only suspends the session and returns to...
... difficulties configuring Interfaces or the routing protocol, refer to the Configuring Host Tables lab Step Login to Router and verify the connection to Router a Login to the Gadsden router b Verify the connection ... ping is not successful, return to step one and troubleshoot the configuration Step Telnet to a remote router a Enter telnet router-name if IP host tables were configured Otherwise, enter the ... both the Serial and the FastEthernet interfaces up? _ Step Suspend the current Telnet session a Enter Ctrl Shift followed by the x key This only suspends the session and returns to...
... steps are completed, logoff, by typing exit, and turn all the devices off Then remove and store the cables and adapter 4-4 CCNA 3: Switching Basics and Intermediate Routing v 3.0 - Lab 7.2.6 Copyright ... command Switch_A#show spanning-tree brief b On Switch_B type show spanning-tree brief at the privileged EXEC mode prompt as follows: Switch_B#show spanning-tree brief Examine the command output and ... brief 3-4 CCNA 3: Switching Basics and Intermediate Routing v 3.0 - Lab 7.2.6 Copyright 2003, Cisco Systems, Inc c What changes have taken place in the command output? On Switch_A? ...
... understandable, and precise Write the business challenge in the space below Duplicate your business challenge on a piece of flip-chart paper Activity 2.3: Identifying the Business Challenge and ... and Vision Statement Exercise 1: Identifying the Business Challenge ! State the business challenge (15 minutes) Participate in small groups as assigned by the instructor Review the Ferguson and ... in the space below You will use this in the next exercise and future activities 12 Activity 2.3: Identifying the Business Challenge and Vision Statement Exercise 2: Developing the Vision Statement...
... Configure the console, virtual terminal and enable passwords Step Configure the interfaces and routing protocol on the Gadsden router a Go to the proper command mode and enter the following: GAD(config)#interface ... terminal and enable passwords Step Configure the interfaces and routing protocol on the Birmingham router a Go to the proper command mode and enter the following: BHM(config)#interface fastethernet ... initial configuration dialog, type N and press Enter When prompted to terminate autoinstall type Y and press Enter Press Enter again 4-7 CCNA 2: Routers and Routing Basics v 3.0 - Lab 3.2.9 Copyright...
... Configure the console, virtual terminal and enable passwords Step Configure the interfaces and routing protocol on the Gadsden router a Go to the proper command mode and enter the following: GAD(config)#interface ... terminal and enable passwords Step Configure the interfaces and routing protocol on the Birmingham router a Go to the proper command mode and enter the following: BHM(config)# interface fastethernet ... N and press Enter When prompted to terminate autoinstall type Y and press Enter Press Enter again b What does the prompt look like? 4-7 CCNA 2: Routers and...
... liver mitochondria (40 mg protein) and incubated with 500 lM mitoDC-81 for h and then cut with ClaI (C and D) DNA was separated by electrophoresis on an agarose gel and the ethidium bromide fluorescence ... bromide gel and the fluorescence recorded (A and C) The DNA was then transferred to a nylon membrane and TPP moieties covalently attached to the DNA were visualized using anti-TPP serum (B and D) The ... binding and selective alkylation leading to a depletion of mtDNA in intact cells (Fig 1) Here we report the synthesis and characterization of a novel mitochondria-targeted alkylating reagent and...
... Kogyo (Osaka, Japan) and 4-aminoresorcinol hydrochloride was from Aldrich (Milwaukee, Wis., USA) DE52 cellulose was from Whatman (Madison, Wis., USA), and DEAE-Cellulofine A-800 and Cellulofine GCL-1000 ... 0.4 g yeast extract and deionized water in L total volume, with the final pH adjusted to pH 6.8 Solution B contained g MgSO4Æ7H2O, and mg each of CaCl2Æ2H2O, CuSO4Æ5H2O, ZnCl2, and FeSO4Æ7H2O, with ... 4-amino-3-hydroxybenzoic acid, 6.0 g Na2HPO4Æ12H2O, and deionized water in 700 mL total volume; the final pH being adjusted to pH 6.8 Solutions A and B were autoclaved, and solution C was sterilized by filtration...
... buffer, pH 7.0, and its UV spectra were recorded between 250 and 190 nm Molecular modelling Structure manipulations and analysis were performed using the PYMOL software [41] Results and Discussion ... of SDTG, modified and unmodified GST I were treated with Woodward’s reagent K and 2,4,6-trinitrobenzenesulfonic acid, and the amount of covalently bound SDTG was determined by subtraction of the ... molecular modelling Table Stoichiometry of SDTG binding to GST I Total carboxy and primary amino groups for the modified and unmodified enzyme were determined by the Woodward’s Reagent K and 2,4,6-trinitrobenzenesulfonic...
... copines-1–6 has shown that copines1, -2 and -3 are ubiquitous, whereas copine-4 has a more restricted distribution in brain, heart and prostate gland, and copine-6 is brain specific [15] Interestingly, ... modulate both their calcium responses and intracellular targeting The C2- and vWA-domains therefore have distinct and crucial roles in the translocation and targeting of the copines Together, ... fulllength copines-2, -3 and -6 prepared for this study (2,3) In addition to truncated versions of copines-2 and -6 containing only specific domains (4–8), domain swaps of copines-2 and -6 (9–11) were...
... the kcat and kcat ⁄ Km values show similar trends as a function of steric hindrance at the 3¢ subsite (Table and Fig 4B) Mutation T249S increases the kcat and kcat ⁄ Km values on 3,5-DMC and 3,6-DMC, ... the kcat values on catechol and 3-MC (Table 1), whereas mutations T249A and T249G reduce by four times the kcat values on catechol and 20 times those measured on 3-MC (Table 1) Thr249 in catechol-2,3-dioxygenase ... C2,3O, and T249G C2,3O All of the mutated proteins were active on catechol (Table 1), and had an iron content similar to that of wild-type C2,3O The regioselectivity of the wild-type and mutant...